ABSTRACT
Objective To explore the relationship between chronic severe hepatitis and cryptosporidium infection to provide evidences for scientific prevention and treatment of chronic severe hepatitis. Methods Fecal samples of 218 patients with chronic severe hepatitis B (CSHB) and 140 children with diarrhea were collected, and were examined for cryptosporidium oocytes by using auramine-phenol staining method (AA-p) and modified acid-fast staining method (MAF), and for cryptosporidium DNA by PCR and restriction digestion analysis. The factors affecting cryptosporidium infection of patients with CSHB were preliminarily analyzed. Results The positive rates of cryptosporidium infection detected by AA-p, MAF and PCR in the patients with CSHB and children with diarrhea were 4.1%, 3.2%, 6.0% and 0.7%, 0.7%, 1.4%, respectively. The positive rate of cryptosporidium infection detected by PCR in patients with CSHB was higher than that in children with diarrhea (P
ABSTRACT
Schistosoma japonicum daughter sporocysts in Oncomelania tissue sections were, for the first time, used as antigen in the diagnosis of schistosomiasis japonica by indirect immunoperoxidase assay (IIPA-DS). The most strong peroxidase reaction was localized on the tegument of the daughter sporocysts and cercariae, and at some parts of cercariaf parenchyma, when IIPA-DS was carried out. Of 112 sera from proven cases of schisto-Bomiasis japonica, 106 (94.6%) were positive, the range of titre dilution was from 1:1 to 1:160, the geometrie mean of titres was 1:20. IIPA-DS highly coincided with IHA, COP and ELJSA in both sensitivity and strength of reaction. Sera from 101 blood donors and 24 normal rabbits were all negative. No cross reaction with sera from 24 patients with paragonimiasis was observed.